As explained by principal scientist Camilla Liscio, PhD, she and her team were intrigued by the fact that Mitra® devices offered an "innovative alternative approach not only tomicrosampling for blood, but also potentially for other biofluids as well (e.g., saliva, urine, sweat)."
Overcoming Biases with Volumetric Absorptive Microsampling
In her article, Dr. Liscio explains that she appreciated that VAMS technology could overcome the uneven analyte distributionchallenges associated with DBS samplesdue to the Hematocrit (HCT) effect. She highlights how Mitra devices are able to absorb a fixed volume of blood (using either 10 μL, 20 μL or 30 μL sized VAMS tips), which eliminates the spot migration and spot punch location biases as previously observed with DBS.
Dr. Liscio reports that, as part of a project for one of their customers, the Element team translated the manual method onto their instrument top X-Y robotic platform. They applied Design of Experiment (DoE) to optimize the automated extraction methodology for the target analytes in whole blood using the Mitra devices, and then proved comparabilitybetween the manual and the automated approach.
The success of that project led the Element Laboratory Solutions team to fully explore the complete automation of the Mitra® sample preparation workflow. They started by trying to answer this question: "Could we automate the delivery of the VAMS tips from thededicated 96-Autorack™ for the Mitra® devices directly into the vial to proceed with the automated extraction as a fully automated workflow?"
This is curated content. Learn more about the work at Element Cambridge to develop and optimize the fully automated workflow for Mitra® handling and extraction for TDM, by following the link below to read the original article and access a free webinar in which Dr. Liscio discusses the automated workflow: https://anatune.co.uk/automated-mitra-for-volumetric-microsampling/