GenTegraRNA-NEO for Microsampling (RUO)

Did you know that RNA can remain stable in a blood sample stored for more than 3 days at room temperature? With the GenTegraRNA-NEO solution, an Active Chemical Protection™ (ACP) technology by GenTegra supplied by Trajan for use on Mitra^® blood microsampling devices, this is now possible! The protection solution arrives as a lyophilized powder that you rehydrate in the lab. It can be applied to VAMS^® tips on Mitra^® devices to stabilize and preserve RNA at ambient temperatures for 7 days prior to extraction for your research applications.

Pharmacogenomics

Sample Stability for 7 Days (Shipping)

RNA Therapy Development

Genetic Determinants of Disease

Disease Pathogenesis

Clinical Trial Screening

Biomarker Discovery

Transcriptomics

Pre-Treat Mitra Devices in the Lab

Treat Microsampling Devices

GenTegraRNA-NEO arrives in your research lab as a dry matrix in a tube. Follow the steps outlined in the GenTegra User Guide to treat the VAMS^® tips of the Mitra^® devices with the rehydrated GenTegraRNA-NEO solution.

When the matrix in the tube is rehydrated, there is sufficient volume to treat 32 VAMS tips, or 8 Mitra devices (4 microsamplers in each device).

You can use the Mitra 96-Autorack™ with 4 tubes of GenTegraRNA-NEO solution to treat 96 Mitra devices in a standard 96 well conical bottom microplate. The treated Mitra devices can be used exactly as you would the untreated Mitra devices.

Benefits for your research applications include:

check-mark-png Quantitative RNA recovery (0.125-0.150 ng)
High-quality RNA (3.0-8.0 RIN)
Easily store & ship treated Mitra devices
RNA-stable for 7 days at ambient temps

Compatible Applications:
check-mark-png tRNA extraction from blood
mRNA, miRNA from blood

Offer Easy, Remote Sample Collection

Finger-Stick Microsampling for RNA Studies

Researchers can offer Mitra^® devices that have been pre-treated with the GenTegraRNA-NEO solution to collect blood microsamples for a range of RNA studies. The treated devices can be used to sample cohorts onsite or remotely with ease.

The GenTegraRNA-NEO solution is typically paired with a 4-sampler Mitra device format featuring 30 µL VAMS tips. The devices arrive in a protective plastic housing that has barcoding for chain of custody.

check-mark-png 4 x 30 µL blood samples
Minimally invasive method
Easy to use for a variety of cohorts
Sample collection for RNA research
Barcoding to manage chain of custody

Enable Stable Shipping, Simple Processing

Samples Remain RNA-Stable for 7 Days

Blood samples collected using the GenTegraRNA-NEO treated Mitra devices are stable at room temperature for up to 7 days. The GenTegraRNA-NEO technology protects against naturally occurring nucleases and environmental stresses that could potentially degrade the RNA sample.

The treated Mitra devices overcome some of the common challenges associated with RNA research, providing benefits that include:

RNA-stable w/out cold shipping or storage

Remote sampling w/out trained staff

Access to diverse & underserved populations
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Rapid processing using 96 well-plates
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Manual processing using standard tubes
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Quality samples generate reliable data

Compatible with standard DBS workflows

Promega Provides App Note for Automated RNA Purification on Trajan Microsampling Devices for RNA-based Studies

Researchers at Promega have published an application note demonstrating a method for purifying Total RNA, including miRNA, from whole blood collected on the VAMS^® tips of Trajan’s Neoteryx Mitra^® devices treated with GenTegraRNA-NEO™ solution using the Promega Maxwell^® RSC simplyRNA Blood Kit and Maxwell^® RSC Instrument.

Cellecta Researchers Profile RNA Biomarkers in Dried Blood Microsamples

Using Mitra^® microsampling devices that were pre-treated with GenTegraRNA-NEO for RNA stabilization before blood collection, Cellecta researchers analyzed the samples with the DriverMap™ Targeted RNA-Seq Expression Profiling Technology 19K Panel and the DriverMap™ T/B Immune Marker Panel to effectively detect up- and down-regulated genes in blood microsamples.