Share this
how to extract DNA from dried blood
by Neoteryx Microsampling on Jun 27, 2018 2:24:00 AM
Did you know that it’s possible to successfully extract DNA/RNA from dried blood spots even after 10 years? There is enough data to support this assertion.
However, the best output can be achieved if storage is done at room temperature using dried samples. As technology evolves, microsampling has emerged as a crucial concept in medicine and healthcare alongside other groundbreaking innovations, representing the next generation of dried blood spot (DBS) technology. Dried blood sampling is an alternative to traditional serum or plasma sampling for pharmaco- or toxicokinetic evaluations that has been applied to diagnostic screening in clinical, nonclinical, and drug discovery settings. Microsampling - combining the best of wet and dry sampling - is the perfect alternative to blood collection tubes that enables efficient, economical, and convenient remote sample collection.
Using a Microsampling Device to Collect Blood
Volumetric Absorptive Microsampling (VAMS) technology involves the use of sophisticated devices including various microsampling apparatus that are instrumental for total RNA/DNA purification and detection. A microsampling device is highly reliable hence you can use it to accurately collect a fixed volume of blood (for instance 10 or 20µL) within seconds. You can easily collect a blood sample through a fingerprick blood draw. You can then dry the sample and transport it to the lab under ambient conditions thus eradicating the need for exorbitant cold chain or biohazardous shipping.
Total DNA/RNA Extraction From Dried Blood
A study conducted to look into the potential of using microsampling devices, based on VAMS technology for total RNA/DNA purification and detection, had much to reveal. On the evaluation, specially optimized protocols based on unique silicon carbide nucleic acid were used to isolate and purify both DNA and RNA from a microsampling device. The researchers used spectrophotometry to measure yields after which they discovered both DNA and RNA (large mRNA and microRNA) had been preserved on the tips of the microsampling device. The isolated DNA/RNA was reported to be of the best quality and all the purified nucleic acids were successfully utilized in various downstream molecular tests such as Single Nucleotide Polymorphism (SNP) analysis.
Alternative blood collection devices indicated above have been shown to be useful in extracting DNA from dried blood. Moreover, they could also be used to counter some of the inconvenience of blood tube collection such as the potential of bodily fluid leakage, the need of venipuncture, and related hassles.
Share this
- Microsampling (186)
- Venipuncture Alternative (108)
- Research, Remote Research (88)
- Clinical Trials, Clinical Research (82)
- Mitra® Device (71)
- Therapeutic Drug Monitoring, TDM (48)
- Dried Blood Spot, DBS (37)
- Biomonitoring, Health, Wellness (35)
- Infectious Disease, Vaccines, COVID-19 (24)
- Decentralized Clinical Trial (DCT) (22)
- Blood Microsampling, Serology (21)
- Omics, Multi-Omics (17)
- Toxicology, Doping, Drug/Alcohol Monitoring, PEth (15)
- hemaPEN® Device (13)
- Preclinical Research, Animal Studies (11)
- Pharmaceuticals, Drug Development (8)
- Harpera® Tool (5)
- Skin Microsampling, Microbiopsy (5)
- Company Press Release, Product Press Release (4)
- Industry News, Microsampling News (4)
- Antibodies, MAbs (3)
- September 2023 (2)
- July 2023 (3)
- June 2023 (2)
- April 2023 (2)
- March 2023 (2)
- February 2023 (2)
- January 2023 (3)
- December 2022 (2)
- November 2022 (3)
- October 2022 (4)
- September 2022 (3)
- August 2022 (5)
- July 2022 (2)
- June 2022 (2)
- May 2022 (4)
- April 2022 (3)
- March 2022 (3)
- February 2022 (4)
- January 2022 (5)
- December 2021 (3)
- November 2021 (5)
- October 2021 (3)
- September 2021 (3)
- August 2021 (4)
- July 2021 (4)
- June 2021 (4)
- May 2021 (4)
- April 2021 (3)
- March 2021 (5)
- February 2021 (4)
- January 2021 (4)
- December 2020 (3)
- November 2020 (5)
- October 2020 (4)
- September 2020 (3)
- August 2020 (3)
- July 2020 (6)
- June 2020 (4)
- May 2020 (4)
- April 2020 (3)
- March 2020 (6)
- February 2020 (3)
- January 2020 (4)
- December 2019 (5)
- November 2019 (6)
- October 2019 (3)
- September 2019 (4)
- August 2019 (5)
- July 2019 (3)
- June 2019 (7)
- May 2019 (6)
- April 2019 (5)
- March 2019 (6)
- February 2019 (5)
- January 2019 (8)
- December 2018 (3)
- November 2018 (4)
- October 2018 (7)
- September 2018 (7)
- August 2018 (5)
- July 2018 (8)
- June 2018 (6)
- May 2018 (5)
- April 2018 (6)
- March 2018 (5)
- February 2018 (6)
- January 2018 (4)
- December 2017 (2)
- November 2017 (3)
- October 2017 (2)
- September 2017 (4)
- August 2017 (2)
- July 2017 (4)
- June 2017 (5)
- May 2017 (6)
- April 2017 (6)
- March 2017 (5)
- February 2017 (4)
- January 2017 (1)
- July 2016 (3)
- May 2016 (1)
- April 2016 (2)
Comments (2)