DNA Collection and Analysis with Dried Blood Microsampling

DNA collection, storage, and analysis play crucial roles in various medical disciplines. Accurate results hinge on obtaining high-quality, stable samples. While fresh blood samples are traditional, they pose challenges in collection, transportation, and storage, often rendering them non-viable.

Dried Blood as a Viable Alternative

Dried blood microsampling has emerged as a practical solution in omics research. It provides stable DNA samples without the logistical constraints of fresh blood. This method circumvents the typical limitations of fresh samples and offers a more flexible approach suitable for diverse research settings.

Sources and Challenges of DNA Extraction

DNA is typically extracted from cheek cells or white blood cells. Cheek cells, while easily accessible, are prone to contamination from external sources. Blood, therefore, remains the preferred DNA source due to its relative purity. However, wet blood requires careful handling to prevent sample degradation and involves complex extraction processes that are not always feasible, especially in resource-limited environments.

Advantages of Dried Blood Microsampling

Dried blood samples alleviate the stringent time and temperature requirements needed for wet blood, allowing for:

• Efficient field collection: Samples can be easily gathered and transported.
• Simplified storage: No refrigeration needed, reducing logistical burdens.
• Enhanced DNA longevity: Studies show that DNA in dried blood remains viable for months, even under challenging conditions like high temperatures or humidity.

Volumetric Absorptive Microsampling Technology

This innovative technology addresses many challenges traditional dried blood spots face on filter paper, which often require labor-intensive extraction processes. Volumetric Absorptive Microsampling simplifies DNA collection, making it less labor-intensive and more suitable for field studies.

Longevity and Stability of Dried Blood DNA

Research confirms that DNA from dried blood spots retains its integrity over extended periods, even when exposed to high humidity or temperatures up to 35°C. Laboratory tests corroborate that there was no significant DNA degradation at 4°C over 24 hours, indicating that extreme cooling is unnecessary for maintaining DNA quality in dried samples.

The advancements in microsampling techniques significantly enhance the viability and accessibility of DNA/RNA collection, especially in non-clinical settings. This method broadens the scope of potential research applications and ensures that genetic analysis remains robust across various environmental conditions.