As more institutions onboard microsampling, the need to automate the handling and extraction of samples is increasing. In this blog we review an article by Caroline Monchaud et al at the CHU Limoges, France that was published in the May 2023 issue of the Journal of Chromatography B.
The paper discusses a successful analytical validation of hemaPEN® blood samples that was achieved for an automated extraction method for tacrolimus measurements using a Shimadzu CLAM-2030® and LCMS-8060®. The hemaPEN device is based on a volumetric microsampling technology that delivers 4 identical dried blood spot (DBS) samples.
Clinical chemistry laboratories are busy places. In many cases, they are processing thousands of blood tubes per day – each containing roughly 5-7 mL of wet blood – for the measurement of a huge variety of analytes, including cardiac and liver panels, electrolytes, drug levels, and more.
Much of the workflow within these laboratories is fully automated, employing barcode scanning that ensures sample identification, from initial sample processing through to the preparation of serum before being sent via conveyor belts (sometimes referred to as "streets") to analyzers. The analyzers utilize techniques such as spectrophotometry, immunoturbidimetry and chemiluminescence immunoassay (CLIA). The data from these instruments is then automatically reported into data systems for review.
A class of molecules which are measured using CLIA are calcineurin inhibitors. These therapeutic drugs are immunosuppressants, which are used in combination with other therapies for graft protection in transplant patients. Although CLIA is used in laboratories for measurement of calcineurin inhibitors, another popular (and sometimes preferred technique), is LC-MS/MS.
Unlike many CLIA analyzers, LC-MS/MS instruments are not fully automated. This often leads to manual sample preparation of blood samples in many laboratories. This is not only time-consuming, but care must be taken to ensure human errors are eliminated in handling and processing the blood samples.
It is hoped that fully automating samples for LC-MS/MS will help minimize errors and free up laboratory technicians for other tasks. Indeed, there are several automation options available for processing blood samples for LC-MS/MS. These include parallel 96 well processing stations as well as serial preparation and direct inject configurations.
Another automation solution, a CLAM-2030 (innovated by Shimadzu), is a fully automated module for sample preparation for biofluids such as blood, urine, and plasma. Like a standard clinical chemistry analyzer, the CLAM-2030 can accept blood tubes and automatically prepare the samples for analysis by LC-MS/MS. At the time of writing, the CLAM-2030 is a research use only product.
Since the COVID-19 Pandemic, the demand for remote capillary microsamples has increased. There has been huge interest in remote microsampling in the field of organ transplantation, where therapeutic drug monitoring (TDM) is critical.
There is an excellent review of microsampling for TDM (including transplant drugs) by Phillip Morgan, who is based at Kings College Hospital London. In a section on the current challenges of automating dried microsamples, Dr. Morgan says, “Small sample substrates must be meticulously extracted from holders, with awkward manipulation in the laboratory; hence, compatibility with automated systems is advantageous.”
To address challenges observed when trying to automate the extraction and analysis of volumetrically collected blood samples, the team at CHU Limoges decided to work on automating dried blood microsamples from hemaPEN devices and interfacing these on a CLAM-2030 connected to a Shimadzu LC-MS/MS.
The hemaPEN is an easy-to-use dried blood microsampling device that collects 4 volumetrically identical capillary samples (2.74µL each) from a single finger-stick. A quick finger-stick method is much less stressful than traditional venous blood draws, making it ideal for therapeutic drug monitoring (TDM) programs.
Once collected, the samples are transferred to a cartridge of 4 dried blood spots (DBS) on pre-punched disks inside the hemaPEN.
The group from CHU Limoges showed a successful validation of the hemaPEN samples through both manual and automation sample extraction protocols for the analysis of tacrolimus.
Furthermore, an excellent correlation between wet and dried sample extracts was observed.
Although biases were seen between wet and dried samples, the differences in the wet extraction method and the dried method may well have contributed. Indeed, we observed something similar around 2015, in a research collaboration with two hospitals in the UK, when we were developing extraction methodology for Cyclosporin (unpublished data). Indeed, by changing the original organic extraction to a method based on the wet method (employing protein precipitation of an aqueous extraction), the resultant data was far more closely aligned.
This study was summarized for our readers by James Rudge, PhD, Technical Director. This is curated content. To learn more about the important research outlined in this blog, visit the original article in the Journal of Chromatography.
More information about microsampling for TDM can be found via our Microsampling for Drug Monitoring page.
Image Credits: Trajan, Neoteryx