October 2020 — Alcohol-related death statistics around the world are staggering. Numbers from the World Health Organization (WHO) reveal that each year, approximately three million people around the globe die as a result of alcohol abuse. That is roughly one in 20 alcohol-related deaths worldwide.
To help address this global health concern, research scientists are investigating ways to help the medical, governmental and law enforcement communities improve alcohol prevention and intervention programs by identifying methods for detecting and quantifying alcohol use and abuse.
The toxicology research team at the Ghent University's Laboratory of Toxicology has provided access to a peer-reviewed article-in-press for the journal Talanta, describing their study of phosphatidylethanol (PEth) as a direct marker of alcohol intake. The Ghent researchers worked with the laboratory research team at the National Institute of Criminalistics and Criminology (NICC) to develop a quantitative method for analyzing PEth in specimen samples as a biomarker of alcohol intake.
For their collaborative PEth study, the research teams in both labs used remote specimen collection as a quick and easy way to gather samples from subjects out in the field, without the need for onsite assistance from healthcare personnel. It is hoped that the collaborative efforts of both labs will lead to a repeatable method for quantifying PEth that can be used by a larger network of labs across Belgium, Europe, and beyond.
The researchers report that once the microsamples arrived in the lab, PEth 16:0/18:1 was quantified after liquid-liquid extraction using two independent isotope dilution – liquid chromatography – tandem mass spectrometry methods.
Investigators at both sites performed a systematic review of the entire process before the final method comparison. For their study, they used samples from 59 routine toxicology cases collected within a one-year time interval.
The two labs solved any initial differences between their methods by concentrating on important methodological aspects: trueness verification of the calibration protocol focusing on the primary material, preparation of the stock solutions, and adequate equilibration of calibrators and QCs. They also focused on verifying comparability of their results, which were obtained with different m/z transitions.
Several of these aspects could only be verified by critically assessing spiked and native samples. After a final validation, they observed good average comparability of the two methods. They report that the average bias was -0.4%, with 85% of the differences within 20%. Moreover, the methods proved to be reproducible and robust within a one-year time interval.
According to the paper, co-authored by Drs. Katleen Van Uytfanghe, Maria del Mar Ramirez Fernandez, Aurelie De Vos, Sarah MR Wille, and Christophe Stove, this study is the first to develop a quantitative volumetric absorptive microsampling method for PEth measurements. Additionally, it is the first study to perform a systematic comparison of PEth measurements between two laboratories.
Learn more about microsampling in toxicology studies by visiting our Toxicology Resources page.