An article by Courtney C. Carignan, et al, at the University of Michigan, Duke University, Colorado School of Mines, and Eurofins USA, was published in the May 2023 edition of Environmental Science & Technology. They outlined the optimization and validation of a method developed at Eurofins USA to monitor environmental toxins in the form of ‘forever chemicals’ comprised of perfluoroalkyl and polyfluoroalkyl substances (PFAS). The paper is entitled “Self-Collection Blood Test for PFASs: Comparing Volumetric Microsamplers with a Traditional Serum Approach.”
So-called 'forever chemicals,' or Perfluoroalkyl and Polyfluoroalkyl Substances (PFAS), are a group (~9000) of organic molecules which contain fluorine atoms as essential moieties of the molecule. They are used in the manufacture of a wide range of commonly used materials. For example, due to their flame- and temperature-resistant properties, they are a key component of firefighters’ foam.
While PFAS chemicals appear to be useful, there is a concern that due to their physicochemical properties, they do not break down easily, if at all. Studies show that PFAS chemicals accumulate in nature and the environment, increasing the likelihood of human exposure, and potential health issues. Indeed, a 2015 study by the Centers for Disease Control and Prevention (CDC) showed that 97% of US citizens have these forever chemicals, or PFAS, in their blood.
According to the CDC, research studies suggests that high concentrations of certain PFAS chemicals may possibly lead to health concerns, such as increased cholesterol levels, changes in liver enzymes, small decreases in infant birth weights, decreased vaccination response in children, risk of preeclampsia or high blood pressure in pregnant women and an increased risk of testicular or kidney cancer.
However, it must be noted that these studies did not investigate the same PFAS chemicals or exposure types, nor did they study identical cohorts. For these reasons, a variety of outcomes have been reported. As a result, there is an interest in conducting large-scale epidemiological and related studies to monitor PFAS levels more systematically in populations.
Using VAMS for Remote PFAS Studies
One of the issues when conducting epidemiological studies to measure environmental contaminants such as PFAS chemicals, is the cost and inconvenience of collecting venous blood samples. If collected at clinics, the venous samples must be shipped on dry ice to specialist laboratories.
One solution to reducing the costs and challenges of blood sample transport is to use remote dried blood sampling in place of venous blood sampling. Dried blood samples overcome the logistical issues associated with collecting and transporting venous blood. Indeed, it is for this reason that the researchers decided to develop a dried blood PFAS assay.
The group first considered dried blood spot (DBS) sampling as it had been used widely in the past, including for PFAS studies. However, the researchers commented that there was a lack of accuracy seen with DBS due to different volumes of blood applied to the filter paper material, which impacted results. This view has been shared in the literature by many other researchers working with DBS samples.
As a result, the researchers decided to use a volumetric absorptive microsampling approach to collecting blood samples. They chose to use Mitra® devices based on VAMS® technology.
In 2018, Neoteryx co-published a study paper with the National Institute for Health and Welfare in Finland which discussed how good linearity, repeatability, accuracy, and stability had been seen for 12 PFAS chemicals using 10 µL Mitra microsamples.
Although the 2018 study demonstrated that PFAS chemicals could be successfully measured from Mitra devices, there were a number of limitations with the work that was presented. The first was the size of the device’s absorptive tip, which limited the volume that could be collected. The second limitation was the size of the cohort (n=12). A third limitation was that serum, a popular matrix for PFAS measurements, had not been collected to allow for assay bridging.
As a result, the researchers embarked on a larger study to see how Mitra would perform in a larger community-based PFAS biomonitoring study. In this larger study, their aim was to compare self-collected Mitra-VAMS microsamples paired with serum harvested from venous collection.
Neoteryx Comments
This is another highly successful example of pairing microsamples with a high-sensitivity multiplex LC-MS/MS assay. This opens up opportunities for remote sampling of hard-to-reach cohorts without the inconvenience and expense of phlebotomy blood draws by trained personnel.
On a separate note, it was interesting to see that greater precision was seen when collecting blood on Mitra devices in the laboratory compared to pipetting. This is not the first time that this has been observed. Indeed, for some applications, Mitra devices could be used to replace pipetting.
For example, when processing plasma samples for LC-MS, plasma could be collected on Mitra devices and dried. Then, on-tip protein precipitation could be conducted with a solvent, such as acetonitrile. This process would thus negate the pipetting and centrifugation steps using a traditional wet plasma workflow. Moreover, if this was conducted using the 96-auto-rack, then 96 samples could be processed simultaneously, improving method throughput.
This article was summarized for our readers by James Rudge, PhD, Microsampling Technical Director. This is curated content. To learn more about the important research outlined in this blog, visit the original article in the journal of Environmental Science & Technology.
Image Credits: Trajan, Neoteryx, iStock