<img height="1" width="1" style="display:none;" alt="" src="https://dc.ads.linkedin.com/collect/?pid=580450&amp;fmt=gif">

application note download

total RNA/DNA purification and detection from blood preserved on a Mitra microsampling device 

 

Norgen-LP-graphic.jpg

The Mitra Microsampler class I medical device is for direct specimen collection of blood and other biological fluids. It is not specific to any clinical test, and is not for use in diagnostic procedures. Use of the Mitra Microsampler in Laboratory Developed Tests (LDTs) requires further processing including the establishment of performance characteristics and successful validation by the laboratory in a manner consistent with CLIA requirements.

This investigation shows that the isolated RNA and DNA were of the highest quality and all nucleic acids purified were successfully used in a variety of downstream molecular tests 

 

Highlights of this research include: 

  • Both total RNA (large mRNA and microRNA) as well total DNA were shown to be preserved in the Mitra device
  • qPCR and Sanger sequencing for DNA as well as RT-qPCR and Next Gen Sequencing for RNA were analyzed